5ZB2

Crystal structure of Rad7 and Elc1 complex in yeast

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA repair protein RAD7	polymer	405	45449.1	1	UniProt (P06779)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
2	B (B)	Elongin-C	polymer	103	11712.0	1	UniProt (Q03071) Pfam (PF03931)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
3	C, D, E, F, G (A)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
4	H (A)	2-(2-METHOXYETHOXY)ETHANOL	non-polymer		120.1	1	Chemie (PG0)
5	I (A)	3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL	non-polymer		326.4	1	Chemie (P33)
6	J (A)	HEXAETHYLENE GLYCOL	non-polymer		282.3	1	Chemie (P6G)
7	K (A)	3,6,9,12,15,18,21,24,27,30,33,36,39,42,45,48,51,54,57-nonadecaoxanonapentacontane-1,59-diol	non-polymer		899.1	1	Chemie (9FO)
8	L (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
9	M, N (A, B)	water	water		18.0	235	Chemie (HOH)

Sequence modifications

A: 165 - 565 (UniProt: P06779)

PDB	External Database	Details
Met 161	-	expression tag
Ala 162	-	expression tag
Arg 163	-	expression tag
Ser 164	-	expression tag

B: 1 - 89 (UniProt: Q03071)

PDB	External Database	Details
Met -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
-	Met 32	deletion
-	Ile 33	deletion
-	Glu 34	deletion
-	Gly 35	deletion
-	Pro 36	deletion
-	Phe 37	deletion
-	Arg 38	deletion
-	Glu 39	deletion
-	Ser 40	deletion
-	Lys 41	deletion

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	57161.1
Non-Polymers*	Number of molecules	10
	Total formula weight	2111.4
All*	Total formula weight	59272.5

*Water molecules are not included.