5Z1B

Structure of Bifidobacterium dentium beta-glucuronidase complexed with coumarin-3-O-glucuronide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Glycosyl hydrolase family 2, TIM barrel domain protein	polymer	692	77131.4	4	UniProt (E0QAF3) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) In PDB	Bifidobacterium dentium ATCC 27679
2	A, B, C, D	beta-D-glucopyranuronic acid	non-polymer		194.1	4	Chemie (BDP)
3	A, B, C, D	3-HYDROXY-2H-CHROMEN-2-ONE	non-polymer		162.1	4	Chemie (SE2)
4		water	water		18.0	2313	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 668 (UniProt: E0QAF3)

PDB	External Database	Details
Met -23	-	initiating methionine
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Gly 0	-	expression tag
Ala 479	Glu 479	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	308525.7
Non-Polymers*	Number of molecules	8
	Total formula weight	1425.1
All*	Total formula weight	309950.8

*Water molecules are not included.