5Z1B
Structure of Bifidobacterium dentium beta-glucuronidase complexed with coumarin-3-O-glucuronide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Glycosyl hydrolase family 2, TIM barrel domain protein | polymer | 692 | 77131.4 | 4 | UniProt (E0QAF3) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) | Bifidobacterium dentium ATCC 27679 | |
| 2 | E, G, I, K (A, B, C, D) | beta-D-glucopyranuronic acid | non-polymer | 194.1 | 4 | Chemie (BDP) | |||
| 3 | F, H, J, L (A, B, C, D) | 3-HYDROXY-2H-CHROMEN-2-ONE | non-polymer | 162.1 | 4 | Chemie (SE2) | |||
| 4 | M, N, O, P (A, B, C, D) | water | water | 18.0 | 2313 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 668 (UniProt: E0QAF3)
| PDB | External Database | Details |
|---|---|---|
| Met -23 | - | initiating methionine |
| His -22 | - | expression tag |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| Ser -16 | - | expression tag |
| Ser -15 | - | expression tag |
| Gly -14 | - | expression tag |
| Val -13 | - | expression tag |
| Asp -12 | - | expression tag |
| Leu -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Thr -9 | - | expression tag |
| Glu -8 | - | expression tag |
| Asn -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Phe -4 | - | expression tag |
| Gln -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Ala 479 | Glu 479 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 308525.7 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1425.1 | |
| All* | Total formula weight | 309950.8 |
