5Z0L

Crystal structure of copper-bound tyrosinase from Streptomyces castaneoglobisporus in complex with the caddie protein obtained by soaking in the hydroxylamine-containing solution for 9 h at 277 K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Tyrosinase	polymer	281	32089.6	1	UniProt (Q83WS2) Pfam (PF00264)	Streptomyces castaneoglobisporus
2	B (B)	MelC	polymer	134	14105.6	1	UniProt (Q83WS1) Pfam (PF06236)	Streptomyces castaneoglobisporus	caddie protein
3	C, D, E, L (A, B)	COPPER (II) ION	non-polymer		63.5	4	Chemie (CU)
4	F (A)	PEROXIDE ION	non-polymer		32.0	1	Chemie (PER)
5	G, H, I, J, K... (A, B)	NITRATE ION	non-polymer		62.0	6	Chemie (NO3)
6	N, O (A, B)	water	water		18.0	460	Chemie (HOH)

Sequence modifications

A: 1 - 273 (UniProt: Q83WS2)

PDB	External Database	Details
Ser 123	Phe 123	conflict
Leu 274	-	expression tag
Glu 275	-	expression tag
His 276	-	expression tag
His 277	-	expression tag
His 278	-	expression tag
His 279	-	expression tag
His 280	-	expression tag
His 281	-	expression tag

B: 1 - 126 (UniProt: Q83WS1)

PDB	External Database	Details
Dah 98	Tyr 98	see sequence details
Leu 127	-	expression tag
Glu 128	-	expression tag
His 129	-	expression tag
His 130	-	expression tag
His 131	-	expression tag
His 132	-	expression tag
His 133	-	expression tag
His 134	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	46195.2
Non-Polymers*	Number of molecules	11
	Total formula weight	658.2
All*	Total formula weight	46853.4

*Water molecules are not included.