5YSH
Diol dehydratase - alpha/T172A mutant complexed with AdoCbl, aerobically-prepared crystal
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D, G, J (A, D, G, J) | Diol dehydrase alpha subunit | polymer | 554 | 60378.1 | 4 | UniProt (Q59470) Pfam (PF02286) | Klebsiella oxytoca | Propanediol dehydratase,Propanediol dehydratase large subunit |
| 2 | B, E, H, K (B, E, H, K) | Diol dehydrase beta subunit | polymer | 200 | 21721.9 | 4 | UniProt (Q59471) Pfam (PF02288) | Klebsiella oxytoca | |
| 3 | C, F, I, L (C, F, I, L) | Diol dehydrase gamma subunit | polymer | 137 | 15611.7 | 4 | UniProt (Q59472) Pfam (PF02287) | Klebsiella oxytoca | Propanediol dehydratase,Propanediol dehydratase small subunit |
| 4 | EA, M, S, Y (J, A, D, G) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 5 | AA, FA, GA, N, O... (G, J, A, D) | POTASSIUM ION | non-polymer | 39.1 | 8 | Chemie (K) | |||
| 6 | BA, HA, P, V (G, J, A, D) | S-1,2-PROPANEDIOL | non-polymer | 76.1 | 4 | Chemie (PGO) | |||
| 7 | CA, IA, Q, W (G, J, A, D) | 5'-DEOXYADENOSINE | non-polymer | 251.2 | 4 | Chemie (5AD) | |||
| 8 | DA, JA, R, X (H, K, B, E) | COBALAMIN | non-polymer | 1330.4 | 4 | Chemie (B12) | |||
| 9 | KA, LA, MA, NA, OA... (A, B, C, D, E...) | water | water | 18.0 | 1300 | Chemie (HOH) |
Sequence modifications
A, D, G, J: 1 - 554 (UniProt: Q59470)
B, E, H, K: 46 - 224 (UniProt: Q59471)
C, F, I, L: 38 - 173 (UniProt: Q59472)
| PDB | External Database | Details |
|---|---|---|
| Ala 172 | Thr 172 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 25 | - | expression tag |
| Ser 26 | - | expression tag |
| Ser 27 | - | expression tag |
| His 28 | - | expression tag |
| His 29 | - | expression tag |
| His 30 | - | expression tag |
| His 31 | - | expression tag |
| His 32 | - | expression tag |
| His 33 | - | expression tag |
| Ser 34 | - | expression tag |
| Ala 35 | - | expression tag |
| Ala 36 | - | expression tag |
| Leu 37 | - | expression tag |
| Glu 38 | - | expression tag |
| Val 39 | - | expression tag |
| Leu 40 | - | expression tag |
| Phe 41 | - | expression tag |
| Gln 42 | - | expression tag |
| Gly 43 | - | expression tag |
| Pro 44 | - | expression tag |
| Gly 45 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 37 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 390846.9 | |
| Non-Polymers* | Number of molecules | 24 |
| Total formula weight | 7103.9 | |
| All* | Total formula weight | 397950.8 |
