5YSH
Diol dehydratase - alpha/T172A mutant complexed with AdoCbl, aerobically-prepared crystal
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D, G, J (A, D, G, J) | Diol dehydrase alpha subunit | polymer | 554 | 60378.1 | 4 | UniProt (Q59470) | Klebsiella oxytoca | Propanediol dehydratase,Propanediol dehydratase large subunit |
| 2 | B, E, H, K (B, E, H, K) | Diol dehydrase beta subunit | polymer | 200 | 21721.9 | 4 | UniProt (Q59471) | Klebsiella oxytoca | |
| 3 | C, F, I, L (C, F, I, L) | Diol dehydrase gamma subunit | polymer | 137 | 15611.7 | 4 | UniProt (Q59472) | Klebsiella oxytoca | Propanediol dehydratase,Propanediol dehydratase small subunit |
| 4 | EA, M, S, Y (J, A, D, G) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) PubChem (271) | |||
| 5 | AA, FA, GA, N, O... (G, J, A, D) | POTASSIUM ION | non-polymer | 39.1 | 8 | Chemie (K) PubChem (813) | |||
| 6 | BA, HA, P, V (G, J, A, D) | S-1,2-PROPANEDIOL | non-polymer | 76.1 | 4 | Chemie (PGO) | |||
| 7 | CA, IA, Q, W (G, J, A, D) | 5'-DEOXYADENOSINE | non-polymer | 251.2 | 4 | Chemie (5AD) PubChem (142) PubChem (71678) PubChem (439182) PubChem (449044) PubChem (1277776) PubChem (6398669) PubChem (6928544) PubChem (12827542) PubChem (16725083) PubChem (23638944) PubChem (25201172) PubChem (25796474) PubChem (25796475) PubChem (40467848) PubChem (23615209) PubChem (40467849) PubChem (676784) PubChem (6541038) PubChem (57115448) PubChem (59731278) PubChem (90321697) PubChem (121275112) PubChem (124363198) PubChem (133636852) PubChem (140689696) PubChem (142801875) PubChem (142801934) | |||
| 8 | DA, JA, R, X (H, K, B, E) | COBALAMIN | non-polymer | 1330.4 | 4 | Chemie (B12) | |||
| 9 | KA, LA, MA, NA, OA... (A, B, C, D, E...) | water | water | 18.0 | 1300 | Chemie (HOH) |
Sequence modifications
A, D, G, J: 1 - 554 (UniProt: Q59470)
B, E, H, K: 46 - 224 (UniProt: Q59471)
C, F, I, L: 38 - 173 (UniProt: Q59472)
| PDB | External Database | Details |
|---|---|---|
| Ala 172 | Thr 172 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 25 | - | expression tag |
| Ser 26 | - | expression tag |
| Ser 27 | - | expression tag |
| His 28 | - | expression tag |
| His 29 | - | expression tag |
| His 30 | - | expression tag |
| His 31 | - | expression tag |
| His 32 | - | expression tag |
| His 33 | - | expression tag |
| Ser 34 | - | expression tag |
| Ala 35 | - | expression tag |
| Ala 36 | - | expression tag |
| Leu 37 | - | expression tag |
| Glu 38 | - | expression tag |
| Val 39 | - | expression tag |
| Leu 40 | - | expression tag |
| Phe 41 | - | expression tag |
| Gln 42 | - | expression tag |
| Gly 43 | - | expression tag |
| Pro 44 | - | expression tag |
| Gly 45 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 37 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 390846.9 | |
| Non-Polymers* | Number of molecules | 24 |
| Total formula weight | 7103.9 | |
| All* | Total formula weight | 397950.8 |
