5YR7

Human methionine aminopeptidase type 1b (F309L mutant)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Methionine aminopeptidase 1	polymer	328	36819.9	1	UniProt (P53582) Pfam (PF00557)	Homo sapiens (Human)	MetAP 1,Peptidase M 1
2	B, C, D, E (A)	COBALT (II) ION	non-polymer		58.9	4	Chemie (CO)
3	F (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
4	G (A)	METHIONINE	non-polymer		149.2	1	Chemie (MET)
5	H (A)	water	water		18.0	70	Chemie (HOH)

Sequence modifications

A: 90 - 395 (UniProt: P53582)

PDB	External Database	Details
Met 68	-	initiating methionine
Gly 69	-	expression tag
Ser 70	-	expression tag
Ser 71	-	expression tag
His 72	-	expression tag
His 73	-	expression tag
His 74	-	expression tag
His 75	-	expression tag
His 76	-	expression tag
His 77	-	expression tag
Ser 78	-	expression tag
Ser 79	-	expression tag
Gly 80	-	expression tag
Leu 81	-	expression tag
Val 82	-	expression tag
Pro 83	-	expression tag
Arg 84	-	expression tag
Gly 85	-	expression tag
Ser 86	-	expression tag
His 87	-	expression tag
Met 88	-	expression tag
Pro 89	-	expression tag
Leu 309	Phe 300	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	36819.9
Non-Polymers*	Number of molecules	6
	Total formula weight	407.9
All*	Total formula weight	37227.8

*Water molecules are not included.