5YR2

Structure of cpGFP66BPA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Green fluorescent protein	polymer	247	27953.4	2	UniProt (A0A059PIQ0)	Aequorea victoria (Jellyfish)
2	C, D (A, B)	water	water		18.0	417	Chemie (HOH)

Sequence modifications

A, B: 2 - 91 (UniProt: A0A059PIQ0)

PDB	External Database	Details
Met 1	-	expression tag
Val 62	Ala 206	engineered mutation

A, B: 100 - 241 (UniProt: A0A059PIQ0)

PDB	External Database	Details
Gly 92	-	linker
Gly 93	-	linker
Thr 94	-	linker
Gly 95	-	linker
Gly 96	-	linker
Ser 97	-	linker
Met 98	-	linker
Ser 99	-	linker
Arg 127	Ser 30	engineered mutation
Bf9 163	Thr 65	chromophore
Bf9 163	Tyr 66	chromophore
Bf9 163	Gly 67	chromophore
Ser 169	Ala 72	engineered mutation
Arg 177	Gln 80	engineered mutation
Leu 242	-	expression tag
Glu 243	-	expression tag
His 244	-	expression tag
His 245	-	expression tag
His 246	-	expression tag
His 247	-	expression tag
His 248	-	expression tag
His 249	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	55906.7
All*	Total formula weight	55906.7

*Water molecules are not included.