5YLZ
Cryo-EM Structure of the Post-catalytic Spliceosome from Saccharomyces cerevisiae at 3.6 angstrom
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Pre-mRNA-splicing factor 8 | polymer | 2413 | 279867.5 | 1 | UniProt (P33334) Pfam (PF08082) Pfam (PF08083) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) Pfam (PF08084) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 2 | B (B) | U5 snRNA | polymer | 214 | 68643.3 | 1 | Saccharomyces cerevisiae S288c (Baker's yeast) | ||
| 3 | C (C) | Pre-mRNA-splicing factor SNU114 | polymer | 1008 | 114174.0 | 1 | UniProt (P36048) Pfam (PF03764) Pfam (PF00679) Pfam (PF16004) Pfam (PF00009) | Saccharomyces cerevisiae S288c (Baker's yeast) | 114 kDa U5 small nuclear ribonucleoprotein component,Growth inhibitory protein 10 |
| 4 | D (D) | U6 snRNA | polymer | 112 | 35883.2 | 1 | Saccharomyces cerevisiae S288c (Baker's yeast) | ||
| 5 | E (E) | mRNA/intron lariat | polymer | 369 | 113649.2 | 1 | Saccharomyces cerevisiae S288c (Baker's yeast) | ||
| 6 | F (F) | U2 snRNA | polymer | 1175 | 376267.4 | 1 | Saccharomyces cerevisiae S288c (Baker's yeast) | ||
| 7 | G (G) | Pre-mRNA-splicing factor SNT309 | polymer | 175 | 20741.5 | 1 | UniProt (Q06091) | Saccharomyces cerevisiae S288c (Baker's yeast) | PRP19-associated complex protein 25,Synergistic to PRP19 mutation protein 309 |
| 8 | H (H) | Pre-mRNA-splicing factor SYF1 | polymer | 859 | 100344.0 | 1 | UniProt (Q04048) Pfam (PF23233) Pfam (PF23220) Pfam (PF23231) | Saccharomyces cerevisiae S288c (Baker's yeast) | PRP19-associated complex protein 90,Synthetic lethal with CDC40 protein 1 |
| 9 | I (I) | Pre-mRNA-splicing factor CLF1 | polymer | 687 | 82555.9 | 1 | UniProt (Q12309) Pfam (PF23233) Pfam (PF23240) | Saccharomyces cerevisiae S288c (Baker's yeast) | Crooked neck-like factor 1,PRP19-associated complex protein 77,Synthetic lethal with CDC40 protein 3 |
| 10 | J (J) | Pre-mRNA-splicing factor CEF1 | polymer | 590 | 67837.8 | 1 | UniProt (Q03654) Pfam (PF00249) Pfam (PF11831) | Saccharomyces cerevisiae S288c (Baker's yeast) | PRP nineteen-associated complex protein 85,PRP19-associated complex protein 85 |
| 11 | K (K) | Pre-mRNA-splicing factor SYF2 | polymer | 215 | 24850.7 | 1 | UniProt (P53277) Pfam (PF08231) | Saccharomyces cerevisiae S288c (Baker's yeast) | PRP19 complex protein 31,Synthetic lethal with CDC40 protein 2 |
| 12 | L (L) | Pre-mRNA-splicing factor BUD31 | polymer | 157 | 18484.5 | 1 | UniProt (P25337) Pfam (PF01125) | Saccharomyces cerevisiae S288c (Baker's yeast) | Bud site selection protein 31,Complexed with CEF1 protein 14 |
| 13 | M (T) | Pre-mRNA-processing factor 17 | polymer | 455 | 52128.8 | 1 | UniProt (P40968) Pfam (PF00400) | Saccharomyces cerevisiae S288c (Baker's yeast) | Cell division control protein 40 |
| 14 | N (M) | Pre-mRNA-splicing factor SLT11 | polymer | 364 | 40988.6 | 1 | UniProt (P38241) Pfam (PF21369) | Saccharomyces cerevisiae S288c (Baker's yeast) | Extracellular mutant protein 2,Synthetic lethality with U2 protein 11 |
| 15 | O (N) | Pre-mRNA-splicing factor CWC2 | polymer | 339 | 38486.6 | 1 | UniProt (Q12046) Pfam (PF16131) Pfam (PF00076) | Saccharomyces cerevisiae S288c (Baker's yeast) | Complexed with CEF1 protein 2,PRP19-associated complex protein 40,Synthetic lethal with CLF1 protein 3 |
| 16 | P (O) | Pre-mRNA-splicing factor PRP46 | polymer | 451 | 50771.3 | 1 | UniProt (Q12417) Pfam (PF00400) | Saccharomyces cerevisiae S288c (Baker's yeast) | Complexed with CEF1 protein 1,PRP nineteen-associated complex protein 50,PRP19-associated complex protein 50,Pre-mRNA-processing protein 46 |
| 17 | Q (P) | Pre-mRNA-splicing factor CWC15 | polymer | 175 | 19975.2 | 1 | UniProt (Q03772) Pfam (PF04889) | Saccharomyces cerevisiae S288c (Baker's yeast) | Complexed with CEF1 protein 15 |
| 18 | R (Q) | Pre-mRNA-processing protein 45 | polymer | 379 | 42548.7 | 1 | UniProt (P28004) Pfam (PF02731) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 19 | S (R) | Pre-mRNA-splicing factor CWC21 | polymer | 135 | 15793.6 | 1 | UniProt (Q03375) Pfam (PF08312) | Saccharomyces cerevisiae S288c (Baker's yeast) | Complexed with CEF1 protein 21 |
| 20 | T (S) | Pre-mRNA-splicing factor CWC22 | polymer | 577 | 67386.1 | 1 | UniProt (P53333) Pfam (PF02847) | Saccharomyces cerevisiae S288c (Baker's yeast) | Complexed with CEF1 protein 22 |
| 21 | U (U) | Pre-mRNA-splicing factor 18 | polymer | 251 | 28414.4 | 1 | UniProt (P33411) Pfam (PF02840) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 22 | V (V) | Pre-mRNA-splicing factor SLU7 | polymer | 382 | 44722.9 | 1 | UniProt (Q02775) Pfam (PF11708) | Saccharomyces cerevisiae S288c (Baker's yeast) | Synthetic lethal with U2 snRNA protein 17,Synthetic lethal with U5 snRNA protein 7 |
| 23 | W (W) | Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP22 | polymer | 1145 | 130187.4 | 1 | UniProt (P24384) Pfam (PF00575) Pfam (PF00270) Pfam (PF00271) Pfam (PF04408) Pfam (PF21010) Pfam (PF07717) | Saccharomyces cerevisiae S288c (Baker's yeast) | |
| 24 | EA, X (a, h) | Small nuclear ribonucleoprotein-associated protein B | polymer | 196 | 22427.0 | 2 | UniProt (P40018) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | snRNP-B,Sm protein B,SmB |
| 25 | FA, Y (b, i) | Small nuclear ribonucleoprotein E | polymer | 94 | 10385.1 | 2 | UniProt (Q12330) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | snRNP-E,Sm protein E,SmE |
| 26 | GA, Z (c, j) | Small nuclear ribonucleoprotein F | polymer | 86 | 9669.9 | 2 | UniProt (P54999) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | snRNP-F,Sm protein F,SmF |
| 27 | AA, HA (d, k) | Small nuclear ribonucleoprotein G | polymer | 77 | 8490.8 | 2 | UniProt (P40204) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | snRNP-G,Sm protein G,SmG |
| 28 | BA, IA (e, l) | Small nuclear ribonucleoprotein Sm D3 | polymer | 101 | 11240.1 | 2 | UniProt (P43321) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | Sm-D3,snRNP core protein D3 |
| 29 | CA, JA (f, m) | Small nuclear ribonucleoprotein Sm D1 | polymer | 146 | 16296.8 | 2 | UniProt (Q02260) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | Sm-D1,snRNP core protein D1 |
| 30 | DA, KA (g, n) | Small nuclear ribonucleoprotein Sm D2 | polymer | 110 | 12876.1 | 2 | UniProt (Q06217) Pfam (PF01423) | Saccharomyces cerevisiae S288c (Baker's yeast) | Sm-D2,snRNP core protein D2 |
| 31 | LA (o) | U2 small nuclear ribonucleoprotein A' | polymer | 238 | 27232.3 | 1 | UniProt (Q08963) Pfam (PF14580) | Saccharomyces cerevisiae S288c (Baker's yeast) | U2 snRNP A',Looks exceptionally like U2A protein 1 |
| 32 | MA (p) | U2 small nuclear ribonucleoprotein B'' | polymer | 111 | 12850.9 | 1 | UniProt (P40567) Pfam (PF13893) | Saccharomyces cerevisiae S288c (Baker's yeast) | U2 snRNP B'' |
| 33 | NA, OA, PA, QA (q, r, s, t) | Pre-mRNA-processing factor 19 | polymer | 503 | 56629.8 | 4 | UniProt (P32523) Pfam (PF08606) | Saccharomyces cerevisiae S288c (Baker's yeast) | RING-type E3 ubiquitin transferase PRP19 |
| 34 | RA (A) | INOSITOL HEXAKISPHOSPHATE | non-polymer | 660.0 | 1 | Chemie (IHP) | |||
| 35 | SA (C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 36 | TA, UA, VA, WA, XA... (C, D) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) PubChem (888) | |||
| 37 | AB, BB, CB, DB, EB... (L, M, N) | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 43 |
| Total formula weight | 2284076.3 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 1721.5 | |
| All* | Total formula weight | 2285797.8 |
