5YKB

The N253F mutant structure of trehalose synthase from Deinococcus radiodurans reveals an open active-site conformation

Replaces:  5GTV

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Trehalose synthase	polymer	571	65020.9	4	UniProt (I3NX86) Pfam (PF00128) Pfam (PF16657)	Deinococcus radiodurans str. R1
2	E, G, I, K (A, B, C, D)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
3	F, H, J, L (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	M, N, O, P (A, B, C, D)	water	water		18.0	237	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 552 (UniProt: I3NX86)

PDB	External Database	Details
Met -1	-	expression tag
Val 0	-	expression tag
Pro 1	-	expression tag
Trp 97	Arg 97	engineered mutation
Phe 253	Asn 253	engineered mutation
Ile 313	Thr 313	engineered mutation
Val 380	Ile 380	engineered mutation
Ser 553	-	expression tag
Arg 554	-	expression tag
Val 555	-	expression tag
Asp 556	-	expression tag
Lys 557	-	expression tag
Leu 558	-	expression tag
Ala 559	-	expression tag
Ala 560	-	expression tag
Ala 561	-	expression tag
Leu 562	-	expression tag
Glu 563	-	expression tag
His 564	-	expression tag
His 565	-	expression tag
His 566	-	expression tag
His 567	-	expression tag
His 568	-	expression tag
His 569	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	260083.7
Non-Polymers*	Number of molecules	8
	Total formula weight	257.5
All*	Total formula weight	260341.2

*Water molecules are not included.