5YFE
Enzymatic and structural characterization of the poly (ethylene terephthalate) hydrolase PETase from I. sakaiensis
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Poly(ethylene terephthalate) hydrolase | polymer | 272 | 28692.7 | 1 | UniProt (A0A0K8P6T7) Pfam (PF01738) | Ideonella sakaiensis (strain 201-F6) | PETase |
| 2 | B, C (A) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 3 | D, E (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 4 | F (A) | water | water | 18.0 | 69 | Chemie (HOH) |
Sequence modifications
A: 1 - 264 (UniProt: A0A0K8P6T7)
| PDB | External Database | Details |
|---|---|---|
| Asn 46 | Thr 72 | engineered mutation |
| Ala 198 | Arg 224 | engineered mutation |
| Asn 261 | Ala 287 | engineered mutation |
| Leu 265 | - | expression tag |
| Glu 266 | - | expression tag |
| His 267 | - | expression tag |
| His 268 | - | expression tag |
| His 269 | - | expression tag |
| His 270 | - | expression tag |
| His 271 | - | expression tag |
| His 272 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 28692.7 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 376.3 | |
| All* | Total formula weight | 29069.0 |
