5YBR

Fe(II)/(alpha)ketoglutarate-dependent dioxygenase PrhA-V150L/A232S in complex with preaustinoid A3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	PrhA	polymer	314	35196.8	2	UniProt (A0A1E1FFL0)	Penicillium brasilianum
2	C, F (A, B)	FE (III) ION	non-polymer		55.8	2	Chemie (FE)
3	D, G (A, B)	2-OXOGLUTARIC ACID	non-polymer		146.1	2	Chemie (AKG)
4	E, H (A, B)	preaustinoid A3	non-polymer		456.5	2	Chemie (8T9)
5	I, J (A, B)	water	water		18.0	176	Chemie (HOH)

Sequence modifications

A, B: 6 - 294 (UniProt: A0A1E1FFL0)

PDB	External Database	Details
Met -15	-	expression tag
Gly -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Ser -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Leu -2	-	expression tag
Val -1	-	expression tag
Pro 0	-	expression tag
Arg 1	-	expression tag
Gly 2	-	expression tag
Ser 3	-	expression tag
His 4	-	expression tag
Met 5	-	expression tag
Leu 150	Val 150	engineered mutation
Ser 232	Ala 232	engineered mutation
Ile 295	-	expression tag
Ser 296	-	expression tag
Ser 297	-	expression tag
Ala 298	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	70393.6
Non-Polymers*	Number of molecules	6
	Total formula weight	1316.9
All*	Total formula weight	71710.6

*Water molecules are not included.