5XZJ

Crystal structure of the Zn-directed tetramer of the engineered cyt cb562 variant, C96T/AB5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Soluble cytochrome b562	polymer	106	11820.2	4	UniProt (P0ABE7) Pfam (PF07361)	Escherichia coli	Cytochrome b-562
2	E, K, P, T (A, B, C, D)	HEME C	non-polymer		618.5	4	Chemie (HEC)
3	F, G, H, I, L... (A, B, C, D)	ZINC ION	non-polymer		65.4	14	Chemie (ZN)
4	J (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5	AA, X, Y, Z (D, A, B, C)	water	water		18.0	288	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 106 (UniProt: P0ABE7)

PDB	External Database	Details
Ala 34	Arg 56	engineered mutation
Ala 38	Leu 60	engineered mutation
Trp 41	Gln 63	engineered mutation
Ser 42	Lys 64	engineered mutation
His 59	Lys 81	engineered mutation
Trp 66	Asp 88	engineered mutation
Ile 69	Val 91	engineered mutation
His 73	Asp 95	engineered mutation
His 77	Lys 99	engineered mutation
Asp 86	Glu 108	engineered mutation
Glu 89	Ala 111	engineered mutation
His 93	Gln 115	engineered mutation
Cys 98	Arg 120	engineered mutation
His 100	Ala 122	engineered mutation
Cys 101	Tyr 123	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	47280.7
Non-Polymers*	Number of molecules	19
	Total formula weight	3425.2
All*	Total formula weight	50705.9

*Water molecules are not included.