5XFW

Crystal structures of FMN-free form of dihydroorotate dehydrogenase from Trypanosoma brucei

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Dihydroorotate dehydrogenase (fumarate)	polymer	334	36463.9	4	UniProt (Q57U83) Pfam (PF01180)	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	DHOdehase,Dihydroorotate oxidase
2	E, F, G, H, I... (A, B, C, D)	MALONATE ION	non-polymer		102.0	15	Chemie (MLI)
3	T, U, V, W (A, B, C, D)	water	water		18.0	780	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 313 (UniProt: Q57U83)

PDB	External Database	Details
Met -20	-	expression tag
Ala -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ala -11	-	expression tag
Ala -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Val -7	-	expression tag
Leu -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Val 115	Ala 115	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	145855.6
Non-Polymers*	Number of molecules	15
	Total formula weight	1530.7
All*	Total formula weight	147386.3

*Water molecules are not included.