5X89

The X-ray crystal structure of subunit fusion RNA splicing endonuclease from Methanopyrus kandleri

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	EndA-like protein,tRNA-splicing endonuclease	polymer	359	41724.1	1	UniProt (Q8TGZ5) UniProt (Q8TGZ7) Pfam (PF02778) Pfam (PF01974)	Methanopyrus kandleri AV19	tRNA-intron endonuclease
2	B, C (A)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
3	D (A)	water	water		18.0	292	Chemie (HOH)

Sequence modifications

A: 14 - 178 (UniProt: Q8TGZ5)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Gly 179	-	linker
Gly 180	-	linker
Gly 181	-	linker

A: 182 - 359 (UniProt: Q8TGZ7)

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	41724.1
Non-Polymers*	Number of molecules	2
	Total formula weight	189.9
All*	Total formula weight	41914.0

*Water molecules are not included.