5X6M

Crystal Structure of SMAD5-MH1 in complex with a composite DNA sequence

Replaces:  4ZL3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, E, H (B, A, E, F)	Mothers against decapentaplegic homolog 5	polymer	150	17322.2	4	UniProt (P97454) Pfam (PF03165)	Mus musculus (Mouse)	Mothers against DPP homolog 5,Dwarfin-C,Dwf-C,SMAD family member 5,mSmad5
2	B, F (C, G)	DNA (5'-D(P*AP*TP*CP*AP*GP*AP*CP*TP*GP*CP*CP*GP*GP*CP*AP*GP*TP*CP*TP*AP*TP*A)-3')	polymer	22	6736.4	2		synthetic construct
3	C, G (D, H)	DNA (5'-D(P*TP*TP*AP*TP*AP*GP*AP*CP*TP*GP*CP*CP*GP*GP*CP*AP*GP*TP*CP*TP*GP*A)-3')	polymer	22	6767.4	2		synthetic construct
4	I, J, K, L (B, A, E, F)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

B, A, E, F: 1 - 143 (UniProt: P97454)

PDB	External Database	Details
Leu 144	-	expression tag
Glu 145	-	expression tag
His 146	-	expression tag
His 147	-	expression tag
His 148	-	expression tag
His 149	-	expression tag
His 150	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	96296.4
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	96558.1

*Water molecules are not included.