5X3I

Kfla1895 D451A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Glycoside hydrolase family 31	polymer	743	82316.6	2	UniProt (D2PPM7) Pfam (PF21568) Pfam (PF13802) Pfam (PF01055) Pfam (PF21365) In PDB	Kribbella flavida DSM 17836
2	A, B	SULFATE ION	non-polymer		96.1	12	Chemie (SO4)
3	A, B	GLYCEROL	non-polymer		92.1	9	Chemie (GOL)
4		water	water		18.0	742	Chemie (HOH)

Sequence modifications

A, B: 1 - 723 (UniProt: D2PPM7)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 451	Asp 451	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	164633.3
Non-Polymers*	Number of molecules	21
	Total formula weight	1981.6
All*	Total formula weight	166614.9

*Water molecules are not included.