5X3F

Crystal structure of the YgjG-Protein A-Zpa963-PKA catalytic domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Putrescine aminotransferase,Immunoglobulin G-binding protein A	polymer	501	54451.4	1	UniProt (P42588) UniProt (P38507) Pfam (PF00202) Pfam (PF02216)	Escherichia coli (strain K12)	Putrescine--2-oxoglutaric acid transaminase,PATase,IgG-binding protein A,Staphylococcal protein A
2	B (B)	Zpa963,cAMP-dependent protein kinase catalytic subunit alpha	polymer	393	45479.8	1	UniProt (P05132) Pfam (PF00069)	Staphylococcus aureus	PKA C-alpha

Sequence modifications

A: 7 - 453 (UniProt: P42588)

PDB	External Database	Details
Gly 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
Met 6	-	expression tag

A: 454 - 503 (UniProt: P38507)

PDB	External Database	Details
Val 456	Asn 222	engineered mutation
Ala 474	Gly 240	engineered mutation

B: 97 - 156 (PDB: 5X3F)
B: 157 - 489 (UniProt: P05132)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	99931.2
All*	Total formula weight	99931.2

*Water molecules are not included.