5X30
Crystal structure of Pseudomonas putida methionine gamma-lyase C116H mutant with L-homocysteine intermediates.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | L-methionine gamma-lyase | polymer | 398 | 42936.7 | 2 | UniProt (P13254) | Pseudomonas putida | MGL,Homocysteine desulfhydrase,L-methioninase |
| 2 | B, D (B, D) | L-methionine gamma-lyase | polymer | 398 | 42708.6 | 2 | UniProt (P13254) | Pseudomonas putida | MGL,Homocysteine desulfhydrase,L-methioninase |
| 3 | E, H (A, C) | 2-AMINO-4-MERCAPTO-BUTYRIC ACID | non-polymer | 135.2 | 2 | Chemie (HCS) | |||
| 4 | F (B) | (2E)-2-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}but-2-enoic acid | non-polymer | 330.2 | 1 | Chemie (4LM) | |||
| 5 | G (B) | HYDROSULFURIC ACID | non-polymer | 34.1 | 1 | Chemie (H2S) | |||
| 6 | I (D) | (2~{S})-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]-4-sulfanyl-butanoic acid | non-polymer | 366.3 | 1 | Chemie (7XF) | |||
| 7 | J, K, L, M (A, B, C, D) | water | water | 18.0 | 1021 | Chemie (HOH) |
Sequence modifications
A, C: 1 - 398 (UniProt: P13254)
B, D: 1 - 398 (UniProt: P13254)
| PDB | External Database | Details |
|---|---|---|
| His 116 | Cys 116 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| His 116 | Cys 116 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 171290.7 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1001.0 | |
| All* | Total formula weight | 172291.7 |
