5X30

Crystal structure of Pseudomonas putida methionine gamma-lyase C116H mutant with L-homocysteine intermediates.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	L-methionine gamma-lyase	polymer	398	42936.7	2	UniProt (P13254) Pfam (PF01053) In PDB	Pseudomonas putida	MGL,Homocysteine desulfhydrase,L-methioninase
2	B, D	L-methionine gamma-lyase	polymer	398	42708.6	2	UniProt (P13254) Pfam (PF01053) In PDB	Pseudomonas putida	MGL,Homocysteine desulfhydrase,L-methioninase
3	A, C	2-AMINO-4-MERCAPTO-BUTYRIC ACID	non-polymer		135.2	2	Chemie (HCS)
4	B	(2E)-2-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}but-2-enoic acid	non-polymer		330.2	1	Chemie (4LM)
5	B	HYDROSULFURIC ACID	non-polymer		34.1	1	Chemie (H2S)
6	D	(2~{S})-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]-4-sulfanyl-butanoic acid	non-polymer		366.3	1	Chemie (7XF)
7		water	water		18.0	1021	Chemie (HOH)

Sequence modifications

A, C: 1 - 398 (UniProt: P13254)

PDB	External Database	Details
His 116	Cys 116	engineered mutation

B, D: 1 - 398 (UniProt: P13254)

PDB	External Database	Details
His 116	Cys 116	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	171290.7
Non-Polymers*	Number of molecules	5
	Total formula weight	1001.0
All*	Total formula weight	172291.7

*Water molecules are not included.