5X22

Crystal structure of Thermus thermophilus transcription initiation complex with GpA and CMPcPP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, K, L	DNA-directed RNA polymerase subunit alpha	polymer	315	35056.2	4	UniProt (Q9Z9H6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (Q5SHR6) In PDB	Thermus thermophilus	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C, M	DNA-directed RNA polymerase subunit beta	polymer	1119	125436.5	2	UniProt (Q8RQE9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D, N	DNA-directed RNA polymerase subunit beta'	polymer	1524	170997.4	2	UniProt (Q8RQE8) Pfam (PF04997) Pfam (PF21668) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E, O	DNA-directed RNA polymerase subunit omega	polymer	99	11533.3	2	UniProt (Q72ID6) Pfam (PF01192) UniProt (by SIFTS) (Q8RQE7) In PDB	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F, P	RNA polymerase sigma factor SigA	polymer	443	50739.4	2	UniProt (Q72L95) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (Q5SKW1) In PDB	Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
6	G, Q	promoter DNA template strand	polymer	21	6480.2	2		Thermus thermophilus
7	H, R	promoter DNA nontemplate strand	polymer	27	8421.4	2		Thermus thermophilus
8	I, S	RNA (5'-R(*GP*A)-3')	polymer	2	629.5	2		Thermus thermophilus
9	F, L, N, P, B...	MAGNESIUM ION	non-polymer		24.3	12	Chemie (MG)
10	N, D	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
11	N, D	5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine	non-polymer		481.2	2	Chemie (2TM)

Sequence modifications

F, P: 1 - 423 (UniProt: Q72L95)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	18
	Total formula weight	888700.0
Non-Polymers*	Number of molecules	18
	Total formula weight	1515.7
All*	Total formula weight	890215.7

*Water molecules are not included.