5X0I
Crystal structure of PKM2 R399E mutant complexed with FBP and serine
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Pyruvate kinase PKM | polymer | 551 | 60160.2 | 4 | UniProt (P14618) Pfam (PF00224) Pfam (PF02887) | Homo sapiens (Human) | Cytosolic thyroid hormone-binding protein,CTHBP,Opa-interacting protein 3,OIP-3,Pyruvate kinase 2/3,Pyruvate kinase muscle isozyme,Thyroid hormone-binding protein 1,THBP1,Tumor M2-PK,p58 |
| 2 | E, J, O, T (A, B, C, D) | 1,6-di-O-phosphono-beta-D-fructofuranose | non-polymer | 340.1 | 4 | Chemie (FBP) PubChem (16219368) PubChem (718) PubChem (24802138) PubChem (10267) PubChem (46937007) PubChem (172313) PubChem (440117) PubChem (89534552) PubChem (445557) PubChem (92209487) PubChem (92209485) PubChem (92209486) PubChem (124383956) PubChem (124540446) PubChem (5702629) PubChem (135053794) PubChem (123134048) PubChem (124202619) PubChem (124427861) PubChem (124524513) PubChem (124604817) PubChem (12800340) PubChem (130233858) PubChem (133126640) PubChem (135055131) PubChem (140434362) PubChem (146159004) PubChem (160510795) | |||
| 3 | F, K, P, U (A, B, C, D) | SERINE | non-polymer | 105.1 | 4 | Chemie (SER) PubChem (617) PubChem (5951) PubChem (71077) | |||
| 4 | G, L, Q, V (A, B, C, D) | PYRUVIC ACID | non-polymer | 88.1 | 4 | Chemie (PYR) PubChem (1060) | |||
| 5 | H, M, R, W (A, B, C, D) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) PubChem (888) | |||
| 6 | I, N, S, X (A, B, C, D) | POTASSIUM ION | non-polymer | 39.1 | 4 | Chemie (K) PubChem (813) | |||
| 7 | AA, BA, Y, Z (C, D, A, B) | water | water | 18.0 | 45 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 531 (UniProt: P14618)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | expression tag |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Glu 399 | Arg 399 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 240640.7 | |
| Non-Polymers* | Number of molecules | 20 |
| Total formula weight | 2386.7 | |
| All* | Total formula weight | 243027.4 |
