5X0I
Crystal structure of PKM2 R399E mutant complexed with FBP and serine
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Pyruvate kinase PKM | polymer | 551 | 60160.2 | 4 | UniProt (P14618) Pfam (PF00224) Pfam (PF02887) In PDB | Homo sapiens (Human) | Cytosolic thyroid hormone-binding protein,CTHBP,Opa-interacting protein 3,OIP-3,Pyruvate kinase 2/3,Pyruvate kinase muscle isozyme,Thyroid hormone-binding protein 1,THBP1,Tumor M2-PK,p58 |
2 | A, B, C, D | 1,6-di-O-phosphono-beta-D-fructofuranose | non-polymer | 340.1 | 4 | Chemie (FBP) | |||
3 | A, B, C, D | SERINE | non-polymer | 105.1 | 4 | Chemie (SER) | |||
4 | A, B, C, D | PYRUVIC ACID | non-polymer | 88.1 | 4 | Chemie (PYR) | |||
5 | A, B, C, D | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
6 | A, B, C, D | POTASSIUM ION | non-polymer | 39.1 | 4 | Chemie (K) | |||
7 | water | water | 18.0 | 45 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 531 (UniProt: P14618)
PDB | External Database | Details |
---|---|---|
Met -19 | - | expression tag |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Glu 399 | Arg 399 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 240640.7 | |
Non-Polymers* | Number of molecules | 20 |
Total formula weight | 2386.7 | |
All* | Total formula weight | 243027.4 |