5WZF

Crystal structure of Mycobacterium tuberculosis VapC20 (Rv2549c), Sarcin-Ricin loop cleaving toxin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	23S rRNA-specific endonuclease VapC20	polymer	144	16073.0	1	UniProt (P95004) Pfam (PF01850)	Mycobacterium tuberculosis H37Rv	Ribonuclease VapC20,RNase VapC20,Toxin VapC20
2	B (B)	23S rRNA-specific endonuclease VapC20	polymer	144	16089.0	1	UniProt (P95004) Pfam (PF01850)	Mycobacterium tuberculosis H37Rv	Ribonuclease VapC20,RNase VapC20,Toxin VapC20
3	C, D (A, B)	water	water		18.0	261	Chemie (HOH)

Sequence modifications

A: 2 - 131 (UniProt: P95004)

PDB	External Database	Details
Met -12	-	initiating methionine
Gly -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Ser -2	-	expression tag
Gln -1	-	expression tag
Ala 0	-	expression tag
Ser 1	-	expression tag

B: 2 - 131 (UniProt: P95004)

PDB	External Database	Details
Met -12	-	initiating methionine
Gly -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Ser -2	-	expression tag
Gln -1	-	expression tag
Ala 0	-	expression tag
Ser 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	32161.9
All*	Total formula weight	32161.9

*Water molecules are not included.