5WU3

Crystal structure of human Tut1 bound with MgUTP, form II

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Speckle targeted PIP5K1A-regulated poly(A) polymerase	polymer	573	62253.6	2	UniProt (Q9H6E5) Pfam (PF22600) Pfam (PF03828)	Homo sapiens (Human)	Star-PAP,RNA-binding motif protein 21,RNA-binding protein 21,U6 snRNA-specific terminal uridylyltransferase 1,U6-TUTase
2	C, G (A, B)	URIDINE 5'-TRIPHOSPHATE	non-polymer		484.1	2	Chemie (UTP)
3	D, H (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	E, I (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	F, J (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
6	K, L (A, B)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

A, B: 141 - 874 (UniProt: Q9H6E5)

PDB	External Database	Details
Met 140	-	initiating methionine
-	Ala 235	deletion
-	Pro 236	deletion
-	Glu 237	deletion
-	Ser 238	deletion
-	Pro 239	deletion
-	Ser 240	deletion
-	Leu 241	deletion
-	Asp 242	deletion
-	Ser 243	deletion
-	Ala 244	deletion
-	Leu 245	deletion
-	Ala 246	deletion
-	Ser 247	deletion
-	Pro 248	deletion
-	Leu 249	deletion
-	Asp 250	deletion
-	Pro 251	deletion
-	Gln 252	deletion
-	Ala 253	deletion
-	Leu 254	deletion
-	Ala 255	deletion
-	Cys 256	deletion
-	Thr 257	deletion
-	Pro 258	deletion
-	Ala 259	deletion
-	Ser 260	deletion
-	Pro 261	deletion
-	Pro 262	deletion
-	Asp 263	deletion
-	Ser 264	deletion
-	Gln 265	deletion
-	Pro 266	deletion
-	Pro 267	deletion
-	Ala 268	deletion
-	Ser 269	deletion
-	Pro 270	deletion
-	Gln 271	deletion
-	Asp 272	deletion
-	Ser 273	deletion
-	Glu 274	deletion
-	Ala 275	deletion
-	Leu 276	deletion
-	Asp 277	deletion
-	Phe 278	deletion
-	Glu 279	deletion
-	Thr 280	deletion
-	Pro 281	deletion
-	Ser 282	deletion
-	Ser 283	deletion
-	Ser 284	deletion
-	Leu 285	deletion
-	Ala 286	deletion
-	Pro 287	deletion
-	Gln 288	deletion
-	Thr 289	deletion
-	Pro 290	deletion
-	Asp 291	deletion
-	Ser 292	deletion
-	Ala 293	deletion
-	Leu 294	deletion
-	Ala 295	deletion
-	Ser 296	deletion
-	Glu 297	deletion
-	Thr 298	deletion
-	Leu 299	deletion
-	Ala 300	deletion
-	Ser 301	deletion
-	Pro 302	deletion
-	Gln 303	deletion
-	Ser 304	deletion
Ala 372	Cys 372	engineered mutation
Ala 399	Cys 399	engineered mutation
Ala 415	Cys 415	engineered mutation
Ala 501	Cys 501	engineered mutation
Ser 504	Cys 504	engineered mutation
Ala 574	Cys 574	engineered mutation
-	Glu 651	deletion
-	Ser 652	deletion
-	Ser 653	deletion
-	Gln 654	deletion
-	Gly 655	deletion
-	Gly 656	deletion
-	Thr 657	deletion
-	Ser 658	deletion
-	Lys 659	deletion
-	Arg 660	deletion
-	Leu 661	deletion
-	Lys 662	deletion
-	Val 663	deletion
-	Asp 664	deletion
-	Gly 665	deletion
-	Gln 666	deletion
-	Lys 667	deletion
-	Asn 668	deletion
-	Cys 669	deletion
-	Cys 670	deletion
-	Glu 671	deletion
-	Glu 672	deletion
-	Gly 673	deletion
-	Lys 674	deletion
-	Glu 675	deletion
-	Glu 676	deletion
-	Gln 677	deletion
-	Gln 678	deletion
-	Gly 679	deletion
-	Cys 680	deletion
-	Ala 681	deletion
-	Gly 682	deletion
-	Asp 683	deletion
-	Gly 684	deletion
-	Gly 685	deletion
-	Glu 686	deletion
-	Asp 687	deletion
-	Arg 688	deletion
-	Val 689	deletion
-	Glu 690	deletion
-	Glu 691	deletion
-	Met 692	deletion
-	Val 693	deletion
-	Ile 694	deletion
-	Glu 695	deletion
-	Val 696	deletion
-	Gly 697	deletion
-	Glu 698	deletion
-	Met 699	deletion
-	Val 700	deletion
-	Gln 701	deletion
-	Asp 702	deletion
-	Trp 703	deletion
-	Ala 704	deletion
-	Met 705	deletion
-	Gln 706	deletion
-	Ser 707	deletion
-	Pro 708	deletion
-	Gly 709	deletion
-	Gln 710	deletion
-	Pro 711	deletion
-	Gly 712	deletion
-	Asp 713	deletion
-	Leu 714	deletion
-	Pro 715	deletion
-	Leu 716	deletion
-	Thr 717	deletion
-	Thr 718	deletion
-	Gly 719	deletion
-	Lys 720	deletion
-	His 721	deletion
-	Gly 722	deletion
-	Ala 723	deletion
-	Pro 724	deletion
-	Gly 725	deletion
-	Glu 726	deletion
-	Glu 727	deletion
-	Gly 728	deletion
-	Gln 729	deletion
-	Pro 730	deletion
-	Ser 731	deletion
-	His 732	deletion
-	Ala 733	deletion
-	Ala 734	deletion
-	Leu 735	deletion
-	Ala 736	deletion
-	Glu 737	deletion
-	Arg 738	deletion
-	Gly 739	deletion
-	Pro 740	deletion
-	Lys 741	deletion
-	Gly 742	deletion
-	His 743	deletion
-	Glu 744	deletion
-	Ala 745	deletion
-	Ala 746	deletion
-	Gln 747	deletion
-	Glu 748	deletion
-	Trp 749	deletion
-	Ser 750	deletion
Leu 875	-	expression tag
Glu 876	-	expression tag
His 877	-	expression tag
His 878	-	expression tag
His 879	-	expression tag
His 880	-	expression tag
His 881	-	expression tag
His 882	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	124507.2
Non-Polymers*	Number of molecules	8
	Total formula weight	1211.9
All*	Total formula weight	125719.1

*Water molecules are not included.