5WU2

Crystal structure of human Tut1 bound with BaUTP, form I

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Speckle targeted PIP5K1A-regulated poly(A) polymerase	polymer	573	62317.7	2	UniProt (Q9H6E5) Pfam (PF22600) Pfam (PF03828) In PDB	Homo sapiens (Human)	Star-PAP,RNA-binding motif protein 21,RNA-binding protein 21,U6 snRNA-specific terminal uridylyltransferase 1,U6-TUTase
2	A, B	URIDINE 5'-TRIPHOSPHATE	non-polymer		484.1	2	Chemie (UTP)
3	A, B	BARIUM ION	non-polymer		137.3	2	Chemie (BA)
4	A, B	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)

Sequence modifications

A, B: 141 - 874 (UniProt: Q9H6E5)

PDB	External Database	Details
Met 140	-	initiating methionine
-	Ala 235	deletion
-	Pro 236	deletion
-	Glu 237	deletion
-	Ser 238	deletion
-	Pro 239	deletion
-	Ser 240	deletion
-	Leu 241	deletion
-	Asp 242	deletion
-	Ser 243	deletion
-	Ala 244	deletion
-	Leu 245	deletion
-	Ala 246	deletion
-	Ser 247	deletion
-	Pro 248	deletion
-	Leu 249	deletion
-	Asp 250	deletion
-	Pro 251	deletion
-	Gln 252	deletion
-	Ala 253	deletion
-	Leu 254	deletion
-	Ala 255	deletion
-	Cys 256	deletion
-	Thr 257	deletion
-	Pro 258	deletion
-	Ala 259	deletion
-	Ser 260	deletion
-	Pro 261	deletion
-	Pro 262	deletion
-	Asp 263	deletion
-	Ser 264	deletion
-	Gln 265	deletion
-	Pro 266	deletion
-	Pro 267	deletion
-	Ala 268	deletion
-	Ser 269	deletion
-	Pro 270	deletion
-	Gln 271	deletion
-	Asp 272	deletion
-	Ser 273	deletion
-	Glu 274	deletion
-	Ala 275	deletion
-	Leu 276	deletion
-	Asp 277	deletion
-	Phe 278	deletion
-	Glu 279	deletion
-	Thr 280	deletion
-	Pro 281	deletion
-	Ser 282	deletion
-	Ser 283	deletion
-	Ser 284	deletion
-	Leu 285	deletion
-	Ala 286	deletion
-	Pro 287	deletion
-	Gln 288	deletion
-	Thr 289	deletion
-	Pro 290	deletion
-	Asp 291	deletion
-	Ser 292	deletion
-	Ala 293	deletion
-	Leu 294	deletion
-	Ala 295	deletion
-	Ser 296	deletion
-	Glu 297	deletion
-	Thr 298	deletion
-	Leu 299	deletion
-	Ala 300	deletion
-	Ser 301	deletion
-	Pro 302	deletion
-	Gln 303	deletion
-	Ser 304	deletion
Ala 372	Cys 372	engineered mutation
Ala 415	Cys 415	engineered mutation
Ala 501	Cys 501	engineered mutation
Ser 504	Cys 504	engineered mutation
-	Glu 651	deletion
-	Ser 652	deletion
-	Ser 653	deletion
-	Gln 654	deletion
-	Gly 655	deletion
-	Gly 656	deletion
-	Thr 657	deletion
-	Ser 658	deletion
-	Lys 659	deletion
-	Arg 660	deletion
-	Leu 661	deletion
-	Lys 662	deletion
-	Val 663	deletion
-	Asp 664	deletion
-	Gly 665	deletion
-	Gln 666	deletion
-	Lys 667	deletion
-	Asn 668	deletion
-	Cys 669	deletion
-	Cys 670	deletion
-	Glu 671	deletion
-	Glu 672	deletion
-	Gly 673	deletion
-	Lys 674	deletion
-	Glu 675	deletion
-	Glu 676	deletion
-	Gln 677	deletion
-	Gln 678	deletion
-	Gly 679	deletion
-	Cys 680	deletion
-	Ala 681	deletion
-	Gly 682	deletion
-	Asp 683	deletion
-	Gly 684	deletion
-	Gly 685	deletion
-	Glu 686	deletion
-	Asp 687	deletion
-	Arg 688	deletion
-	Val 689	deletion
-	Glu 690	deletion
-	Glu 691	deletion
-	Met 692	deletion
-	Val 693	deletion
-	Ile 694	deletion
-	Glu 695	deletion
-	Val 696	deletion
-	Gly 697	deletion
-	Glu 698	deletion
-	Met 699	deletion
-	Val 700	deletion
-	Gln 701	deletion
-	Asp 702	deletion
-	Trp 703	deletion
-	Ala 704	deletion
-	Met 705	deletion
-	Gln 706	deletion
-	Ser 707	deletion
-	Pro 708	deletion
-	Gly 709	deletion
-	Gln 710	deletion
-	Pro 711	deletion
-	Gly 712	deletion
-	Asp 713	deletion
-	Leu 714	deletion
-	Pro 715	deletion
-	Leu 716	deletion
-	Thr 717	deletion
-	Thr 718	deletion
-	Gly 719	deletion
-	Lys 720	deletion
-	His 721	deletion
-	Gly 722	deletion
-	Ala 723	deletion
-	Pro 724	deletion
-	Gly 725	deletion
-	Glu 726	deletion
-	Glu 727	deletion
-	Gly 728	deletion
-	Gln 729	deletion
-	Pro 730	deletion
-	Ser 731	deletion
-	His 732	deletion
-	Ala 733	deletion
-	Ala 734	deletion
-	Leu 735	deletion
-	Ala 736	deletion
-	Glu 737	deletion
-	Arg 738	deletion
-	Gly 739	deletion
-	Pro 740	deletion
-	Lys 741	deletion
-	Gly 742	deletion
-	His 743	deletion
-	Glu 744	deletion
-	Ala 745	deletion
-	Ala 746	deletion
-	Gln 747	deletion
-	Glu 748	deletion
-	Trp 749	deletion
-	Ser 750	deletion
Leu 875	-	expression tag
Glu 876	-	expression tag
His 877	-	expression tag
His 878	-	expression tag
His 879	-	expression tag
His 880	-	expression tag
His 881	-	expression tag
His 882	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	124635.4
Non-Polymers*	Number of molecules	6
	Total formula weight	1313.8
All*	Total formula weight	125949.3

*Water molecules are not included.