5WN2

APE1 exonuclease substrate complex with phosphoglycolate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA-(apurinic or apyrimidinic site) lyase	polymer	276	31154.5	2	UniProt (P27695) Pfam (PF03372) In PDB	Homo sapiens (Human)	APEX1, APEX nuclease,APEN,Apurinic-apyrimidinic endonuclease 1, APE-1, REF-1, Redox factor-1
2	C	DNA (5'-D(P*TP*CP*GP*AP*CP*GP*GP*AP*TP*CP*C)-3')	polymer	11	3334.2	1		synthetic construct
3	D	DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*G)-3')	polymer	9	2771.8	1		synthetic construct
4	E	DNA (5'-D(*GP*GP*AP*TP*CP*CP*GP*TP*CP*GP*AP*CP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3')	polymer	21	6409.1	1		synthetic construct
5	A, E	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
6	B, C	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
7	D	2-PHOSPHOGLYCOLIC ACID	non-polymer		156.0	1	Chemie (PGA)
8	E	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
9		water	water		18.0	243	Chemie (HOH)

Sequence modifications

A, B: 43 - 318 (UniProt: P27695)

PDB	External Database	Details
Gln 96	Glu 96	engineered mutation
Ala 138	Cys 138	engineered mutation
Asn 210	Asp 210	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	74824.2
Non-Polymers*	Number of molecules	9
	Total formula weight	530.4
All*	Total formula weight	75354.6

*Water molecules are not included.