5WGB

Crystal Structure of the Human mitochondrial Cysteine Desulfurase in complex with ISD11 and E. coli ACP1 protein at 2.75A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cysteine desulfurase, mitochondrial	polymer	426	47395.2	1	UniProt (Q9Y697) Pfam (PF00266)	Homo sapiens (Human)
2	B (B)	LYR motif-containing protein 4	polymer	91	10763.5	1	UniProt (Q9HD34) Pfam (PF05347)	Homo sapiens (Human)
3	C (C)	Acyl carrier protein	polymer	77	8514.3	1	UniProt (B7MJ81) Pfam (PF00550)	Escherichia coli O45:K1 (strain S88 / ExPEC)	ACP
4	D (A)	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	1	Chemie (PLP)
5	E (C)	S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate	non-polymer		540.7	1	Chemie (8Q1)

Sequence modifications

A: 56 - 457 (UniProt: Q9Y697)

PDB	External Database	Details
Met 32	-	initiating methionine
Gly 33	-	expression tag
Ser 34	-	expression tag
Ser 35	-	expression tag
His 36	-	expression tag
His 37	-	expression tag
His 38	-	expression tag
His 39	-	expression tag
His 40	-	expression tag
His 41	-	expression tag
Ser 42	-	expression tag
Ser 43	-	expression tag
Gly 44	-	expression tag
Leu 45	-	expression tag
Val 46	-	expression tag
Pro 47	-	expression tag
Arg 48	-	expression tag
Gly 49	-	expression tag
Ser 50	-	expression tag
His 51	-	expression tag
Met 52	-	expression tag
Leu 53	-	expression tag
Glu 54	-	expression tag
Met 55	-	expression tag

B: 1 - 91 (UniProt: Q9HD34)

PDB	External Database	Details
Ala 11	Ser 11	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	66672.9
Non-Polymers*	Number of molecules	2
	Total formula weight	787.8
All*	Total formula weight	67460.7

*Water molecules are not included.