5WF7

Chaetomium thermophilum Polycomb Repressive Complex 2 bound to GSK126

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Polycomb Protein EED	polymer	605	66021.4	1	UniProt (G0S8H7) Pfam (PF00400) Pfam (PF22038) In PDB	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
2	B	Histone-lysine-N-methyltransferase EZH2, Polycomb protein SUZ12 chimera	polymer	937	106869.6	1	UniProt (G0SDW4) UniProt (G0RYC6) Pfam (PF18601) Pfam (PF18600) Pfam (PF21509) Pfam (PF00856) Pfam (PF09733) In PDB	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)	Zinc finger domain-containing protein
3	B	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
4	B	1-[(2S)-butan-2-yl]-N-[(4,6-dimethyl-2-oxo-1,2-dihydropyridin-3-yl)methyl]-3-methyl-6-[6-(piperazin-1-yl)pyridin-3-yl]-1H-indole-4-carboxamide	non-polymer		526.7	1	Chemie (A9G)
5		water	water		18.0	288	Chemie (HOH)

Sequence modifications

A: 1 - 565 (UniProt: G0S8H7)

PDB	External Database	Details
Met -39	-	initiating methionine
Ala -38	-	expression tag
Ser -37	-	expression tag
Ala -36	-	expression tag
Trp -35	-	expression tag
Ser -34	-	expression tag
His -33	-	expression tag
Pro -32	-	expression tag
Gln -31	-	expression tag
Phe -30	-	expression tag
Glu -29	-	expression tag
Lys -28	-	expression tag
Gly -27	-	expression tag
Gly -26	-	expression tag
Gly -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Gly -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ala -16	-	expression tag
Trp -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
Pro -12	-	expression tag
Gln -11	-	expression tag
Phe -10	-	expression tag
Glu -9	-	expression tag
Lys -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

B: 191 - 2523 (UniProt: G0SDW4)

PDB	External Database	Details
Ser 182	-	expression tag
Asn 183	-	expression tag
His 184	-	expression tag
His 185	-	expression tag
His 186	-	expression tag
His 187	-	expression tag
His 188	-	expression tag
His 189	-	expression tag
Ala 190	-	expression tag
Leu 2524	-	linker
Val 2525	-	linker
Pro 2526	-	linker
Arg 2527	-	linker
Gly 2528	-	linker
Ser 2529	-	linker

B: 2530 - 2691 (UniProt: G0RYC6)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	172891.0
Non-Polymers*	Number of molecules	9
	Total formula weight	1049.9
All*	Total formula weight	173940.9

*Water molecules are not included.