5WEF

Crystal structure of the influenza virus PA endonuclease (F105S mutant) in complex with inhibitor 7a (SRI-29770)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Polymerase acidic protein	polymer	187	21910.1	1	UniProt (C3W5S0)	Influenza A virus
2	B, C (A)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
3	D (A)	2-[(2S)-1-(2,6-dichlorobenzene-1-carbonyl)pyrrolidin-2-yl]-5-hydroxy-6-oxo-N-(2-phenylethyl)-1,6-dihydropyrimidine-4-carboxamide	non-polymer		501.4	1	Chemie (GY6)
4	E, F, G (A)	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
5	H (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
6	I (A)	water	water		18.0	60	Chemie (HOH)

Sequence modifications

A: 1 - 50 (UniProt: C3W5S0)
A: 73 - 196 (UniProt: C3W5S0)

PDB	External Database	Details
Gly 51	-	linker
Gly 52	-	linker
Ser 53	-	linker
Ser 105	Phe 105	engineered mutation
Ala 197	-	expression tag
Ala 198	-	expression tag
Ala 199	-	expression tag
Glu 200	-	expression tag
Leu 201	-	expression tag
Ala 202	-	expression tag
Leu 203	-	expression tag
Val 204	-	expression tag
Pro 205	-	expression tag
Arg 206	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	21910.1
Non-Polymers*	Number of molecules	7
	Total formula weight	820.4
All*	Total formula weight	22730.5

*Water molecules are not included.