5WCZ

Crystal Structure of Wild-Type MalL from Bacillus subtilis with TS analogue 1-deoxynojirimycin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Oligo-1,6-glucosidase 1	polymer	586	69235.4	2	UniProt (O06994) Pfam (PF00128) Pfam (PF16657)	Bacillus subtilis	Dextrin 6-alpha-D-glucanohydrolase,Oligosaccharide alpha-1,6-glucosidase 1,Sucrase-isomaltase 1,Isomaltase 1
2	C, F (A, B)	1-DEOXYNOJIRIMYCIN	non-polymer		163.2	2	Chemie (NOJ)
3	D, E, G (A, B)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
4	H, I (A, B)	water	water		18.0	1266	Chemie (HOH)

Sequence modifications

A, B: 1 - 561 (UniProt: O06994)

PDB	External Database	Details
Met -24	-	initiating methionine
Ser -23	-	expression tag
Tyr -22	-	expression tag
Tyr -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Ile -11	-	expression tag
Pro -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
Gly 2	Ser 2	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	138470.9
Non-Polymers*	Number of molecules	5
	Total formula weight	602.6
All*	Total formula weight	139073.5

*Water molecules are not included.