5W7N
2-Se-T2/4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | RNA (5'-R(*UP*CP*GP*AP*CP*A)-3') | polymer | 6 | 1875.2 | 1 | synthetic construct | ||
| 2 | B (C) | DNA (5'-D(*AP*(US3)P*GP*(US3)P*CP*G)-3') | polymer | 6 | 1950.2 | 1 | synthetic construct | ||
| 3 | C (A) | Ribonuclease H | polymer | 132 | 15243.2 | 1 | UniProt (Q9KEI9) | Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) | RNase H |
| 4 | D, E (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 5 | F, G, H (B, C, A) | water | water | 18.0 | 48 | Chemie (HOH) |
Sequence modifications
A: 62 - 193 (UniProt: Q9KEI9)
| PDB | External Database | Details |
|---|---|---|
| Asn 132 | Asp 132 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 19068.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 48.6 | |
| All* | Total formula weight | 19117.2 |
