5W6Q
Structural basis for recognition of artificial DNA by an evolved KlenTaq variant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, G | DNA polymerase I, thermostable | polymer | 540 | 60862.9 | 3 | UniProt (P19821) Pfam (PF09281) Pfam (PF00476) In PDB | Thermus aquaticus | Taq polymerase 1 |
| 2 | B, E, H | DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(1W5))-3') | polymer | 12 | 3677.4 | 3 | Escherichia coli | ||
| 3 | D, F, I | DNA (5'-D(P*GP*(1WA)P*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3') | polymer | 13 | 4026.6 | 3 | Escherichia coli | ||
| 4 | water | water | 18.0 | 295 | Chemie (HOH) |
Sequence modifications
A, C, G: 293 - 832 (UniProt: P19821)
| PDB | External Database | Details |
|---|---|---|
| Val 444 | Met 444 | engineered mutation |
| Ala 527 | Pro 527 | engineered mutation |
| Glu 551 | Asp 551 | engineered mutation |
| Val 832 | Glu 832 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 205700.7 | |
| All* | Total formula weight | 205700.7 |
