5W6K
Structure of mutant Taq Polymerase incorporating unnatural base pairs Z:P
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase I, thermostable | polymer | 540 | 60862.9 | 1 | UniProt (P19821) | Thermus aquaticus | Taq polymerase 1 |
| 2 | B (B) | DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*GP*CP*(DOC))-3') | polymer | 12 | 3617.4 | 1 | Escherichia coli | ||
| 3 | C (C) | DNA (5'-D(P*(1WA)P*GP*GP*CP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3') | polymer | 13 | 4026.6 | 1 | Escherichia coli | ||
| 4 | D (A) | (1R)-1-[6-amino-5-(dihydroxyamino)-2-hydroxypyridin-3-yl]-1,4-anhydro-2-deoxy-5-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl]-D-erythro-pentitol | non-polymer | 513.2 | 1 | Chemie (A5J) | |||
| 5 | E, F (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | G, H, I (A, B, C) | water | water | 18.0 | 167 | Chemie (HOH) |
Sequence modifications
A: 293 - 832 (UniProt: P19821)
| PDB | External Database | Details |
|---|---|---|
| Val 444 | Met 444 | engineered mutation |
| Ala 527 | Pro 527 | engineered mutation |
| Glu 551 | Asp 551 | engineered mutation |
| Val 832 | Glu 832 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 68506.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 561.8 | |
| All* | Total formula weight | 69068.7 |
