5W5N

Crystal structure of human IgG4-Sigma2 Fc fragment

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Immunoglobulin heavy constant gamma 4	polymer	222	24973.1	2	UniProt (P01861) Pfam (PF07654)	Homo sapiens (Human)	Ig gamma-4 chain C region
2	C (C)	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1479.3	1	In PDB GlyTouCan (G89993FE)
3	D (D)	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1625.5	1	In PDB GlyTouCan (G27919IH)
4	E, G (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	F (B)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
6	H, I (A, B)	water	water		18.0	267	Chemie (HOH)

Sequence modifications

A, B: 227 - 447 (UniProt: P01861)

PDB	External Database	Details
Thr 226	-	expression tag
Pro 229	Ser 108	engineered mutation
Ala 235	Phe 114	engineered mutation
Ala 236	Leu 115	engineered mutation
-	Gly 116	deletion
Ala 237	Gly 117	engineered mutation
Ser 238	Pro 118	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	49946.2
Branched	Number of molecules	2
	Total formula weight	3104.8
Non-Polymers*	Number of molecules	3
	Total formula weight	243.2
All*	Total formula weight	53294.3

*Water molecules are not included.