5VZW

TRIM23 RING domain in complex with UbcH5-Ub

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, B)	Ubiquitin-conjugating enzyme E2 D2	polymer	147	16851.4	2	UniProt (P62837) Pfam (PF00179)	Homo sapiens (Human)	(E3-independent) E2 ubiquitin-conjugating enzyme D2, E2 ubiquitin-conjugating enzyme D2, Ubiquitin carrier protein D2, Ubiquitin-conjugating enzyme E2(17)KB 2, Ubiquitin-conjugating enzyme E2-17 kDa 2, Ubiquitin-protein ligase D2, p53-regulated ubiquitin-conjugating enzyme 1
2	B, F (C, D)	Ubiquitin	polymer	79	8859.1	2	UniProt (P0CG47) Pfam (PF00240)	Homo sapiens (Human)
3	C, D (F, G)	E3 ubiquitin-protein ligase TRIM23	polymer	123	13106.1	2	UniProt (P36406) Pfam (PF13445)	Homo sapiens (Human)	ADP-ribosylation factor domain-containing protein 1, GTP-binding protein ARD-1, RING finger protein 46, RING-type E3 ubiquitin transferase TRIM23, Tripartite motif-containing protein 23
4	G, H, I, J (F, G)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5	K, L, M, N, O... (A, C, F, G, B...)	water	water		18.0	51	Chemie (HOH)

Sequence modifications

A, B: 1 - 147 (UniProt: P62837)

PDB	External Database	Details
Arg 22	Ser 22	engineered mutation
Lys 85	Cys 85	engineered mutation

C, D: 1 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	77633.1
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	77894.7

*Water molecules are not included.