5VZ0
Crystal structure of Lactococcus lactis pyruvate carboxylase G746A mutant in complex with cyclic-di-AMP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Pyruvate carboxylase | polymer | 1144 | 127433.8 | 4 | UniProt (A0A089XIW4) | Lactococcus lactis | |
| 2 | E, I, L, N (A, B, C, D) | MANGANESE (II) ION | non-polymer | 54.9 | 4 | Chemie (MN) | |||
| 3 | F, J, O (A, B, D) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 3 | Chemie (ADP) | |||
| 4 | G, K, P (A, B, D) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 5 | H, M (B, C) | (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8 ]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide | non-polymer | 658.4 | 2 | Chemie (2BA) | |||
| 6 | Q, R, S, T (A, B, C, D) | water | water | 18.0 | 1217 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 1137 (UniProt: A0A089XIW4)
| PDB | External Database | Details |
|---|---|---|
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 746 | Gly 746 | engineered mutation |
| Ala 1055 | Thr 1055 | variant |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 509735.0 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 2891.1 | |
| All* | Total formula weight | 512626.1 |
