5VYU
Crystal structure of the WbkC N-formyltransferase from Brucella melitensis in complex with GDP-perosaminea and N-10-formyltetrahydrofolate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Gdp-mannose 4,6-dehydratase / gdp-4-amino-4,6-dideoxy-d-mannose formyltransferase | polymer | 261 | 29235.6 | 2 | UniProt (F8WJP6) Pfam (PF00551) | Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) | |
| 2 | C, E (A, B) | N-{4-[{[(6S)-2-amino-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}(formyl)amino]benzoyl}-L-glutamic acid | non-polymer | 473.4 | 2 | Chemie (1YA) | |||
| 3 | D (A) | GDP-perosamine | non-polymer | 588.4 | 1 | Chemie (JB2) | |||
| 4 | F (B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 5 | G, H (A, B) | water | water | 18.0 | 173 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 259 (UniProt: F8WJP6)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 78 | Asp 78 | engineered mutation |
| Ala 142 | Phe 142 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 58471.1 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1978.4 | |
| All* | Total formula weight | 60449.5 |
