5VVV

Structural Investigations of the Substrate Specificity of Human O-GlcNAcase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Protein O-GlcNAcase	polymer	504	57822.6	2	UniProt (O60502) Pfam (PF07555) In PDB	Homo sapiens (Human)	OGA,Beta-N-acetylglucosaminidase,Beta-N-acetylhexosaminidase,Beta-hexosaminidase,Meningioma-expressed antigen 5,N-acetyl-beta-D-glucosaminidase,N-acetyl-beta-glucosaminidase,Nuclear cytoplasmic O-GlcNAcase and acetyltransferase,NCOAT
2	B, D	a-crystallin B	polymer	13	1516.7	2	UniProt (by SIFTS) (P02511) In PDB	Homo sapiens
3	B, D	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
4		water	water		18.0	35	Chemie (HOH)

Sequence modifications

A, C: 60 - 542 (UniProt: O60502)

PDB	External Database	Details
His 59	-	expression tag
Asn 175	Asp 175	engineered mutation

A, C: 553 - 704 (UniProt: O60502)

PDB	External Database	Details
Gly 543	-	linker
Gly 544	-	linker
Gly 545	-	linker
Gly 546	-	linker
Ser 547	-	linker
Gly 548	-	linker
Gly 549	-	linker
Gly 550	-	linker
Gly 551	-	linker
Ser 552	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	118678.6
Non-Polymers*	Number of molecules	2
	Total formula weight	442.4
All*	Total formula weight	119121.0

*Water molecules are not included.