5VTX

Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin G225M/L226T/S228A mutant apo form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E	Hemagglutinin HA1 chain	polymer	323	35553.0	3	UniProt (Q91MA7) Pfam (PF00509) In PDB	Influenza A virus (strain A/Hong Kong/1/1968 H3N2)
2	B, D, F	Hemagglutinin HA2 chain	polymer	174	20041.1	3	UniProt (Q91MA7) Pfam (PF00509) In PDB	Influenza A virus (strain A/Hong Kong/1/1968 H3N2)
3	G, I	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
4	H, J, L	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
5	K	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G81315DD)
6	A, C, D, E, F	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	7	Chemie (NAG)
7		water	water		18.0	256	Chemie (HOH)

Sequence modifications

A, C, E: 11 - 329 (UniProt: Q91MA7)

PDB	External Database	Details
Ala 7	-	expression tag
Asp 8	-	expression tag
Pro 9	-	expression tag
Gly 10	-	expression tag
Met 225	Gly 241	engineered mutation
Thr 226	Leu 242	engineered mutation
Ala 228	Ser 244	engineered mutation

B, D, F: 1 - 174 (UniProt: Q91MA7)

PDB	External Database	Details
Gly 123	Arg 468	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	166782.5
Branched	Number of molecules	6
	Total formula weight	3195.0
Non-Polymers*	Number of molecules	7
	Total formula weight	1548.5
All*	Total formula weight	171525.9

*Water molecules are not included.