5VTR

Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin G225L/L226S mutant in complex with 6'-SLN

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E	Hemagglutinin HA1 chain	polymer	323	35537.0	3	UniProt (Q91MA7) Pfam (PF00509) In PDB	Influenza A virus (strain A/Hong Kong/1/1968 H3N2)
2	B, D, F	Hemagglutinin HA2 chain	polymer	174	20041.1	3	UniProt (Q91MA7) Pfam (PF00509) In PDB	Influenza A virus (strain A/Hong Kong/1/1968 H3N2)
3	G, K	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
4	H, J, L, N	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
5	I	N-acetyl-alpha-neuraminic acid-(2-6)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		674.6	1	In PDB BIRD (PRD_900046) GlyTouCan (G73578JC)		6'-sialyl-N-acetyllactosamine
6	M	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G22768VO)
7	A, C, E	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
8	C, E	N-acetyl-alpha-neuraminic acid	non-polymer		309.3	2	Chemie (SIA)
9		water	water		18.0	374	Chemie (HOH)

Sequence modifications

A, C, E: 11 - 329 (UniProt: Q91MA7)

PDB	External Database	Details
Ala 7	-	expression tag
Asp 8	-	expression tag
Pro 9	-	expression tag
Gly 10	-	expression tag
Leu 225	Gly 241	engineered mutation
Ser 226	Leu 242	engineered mutation

B, D, F: 1 - 174 (UniProt: Q91MA7)

PDB	External Database	Details
Gly 123	Arg 468	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	166734.3
Branched	Number of molecules	8
	Total formula weight	4456.1
Non-Polymers*	Number of molecules	6
	Total formula weight	1503.4
All*	Total formula weight	172693.8

*Water molecules are not included.