5VPX

I38T mutant of 2009 H1N1 PA Endonuclease in complex with RO-7

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Polymerase acidic protein	polymer	197	23136.3	1	UniProt (C3W5S0) In PDB	Influenza A virus
2	A	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
3	A	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	A	1-[(11S)-6,11-dihydrodibenzo[b,e]thiepin-11-yl]-5-hydroxy-3-[(2R)-1,1,1-trifluoropropan-2-yl]-2,3-dihydro-1H-pyrido[2,1-f][1,2,4]triazine-4,6-dione	non-polymer		487.5	1	Chemie (R07)
5		water	water		18.0	8	Chemie (HOH)

Sequence modifications

A: 1 - 53 (UniProt: C3W5S0)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Thr 38	Ile 38	engineered mutation
Gly 51	Phe 51	linker
Gly 52	His 52	linker
Ser 53	Phe 53	linker

A: 73 - 196 (UniProt: C3W5S0)

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	23136.3
Non-Polymers*	Number of molecules	5
	Total formula weight	789.5
All*	Total formula weight	23925.8

*Water molecules are not included.