5VOB

Crystal structure of HCMV Pentamer in complex with neutralizing antibody 8I21

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Envelope glycoprotein H	polymer	725	82415.9	1	UniProt (Q6SW67) Pfam (PF02489) Pfam (PF17488)	Human cytomegalovirus (HHV-5)	gH
2	B (B)	Envelope glycoprotein L	polymer	278	30846.5	1	UniProt (Q68674) Pfam (PF01801)	Human cytomegalovirus (strain 5508) (HHV-5)	gL
3	C (C)	Envelope glycoprotein UL128	polymer	171	19777.0	1	UniProt (P16837)	Human cytomegalovirus (strain AD169) (HHV-5)
4	D (D)	Envelope glycoprotein UL130	polymer	252	28664.6	1	UniProt (F5HCP3) Pfam (PF11668)	Human cytomegalovirus (strain Merlin) (HHV-5)
5	E (E)	Envelope glycoprotein UL131A	polymer	129	15011.0	1	UniProt (F5HET4)	Human cytomegalovirus (strain Merlin) (HHV-5)
6	F (H)	Fab 8I21 heavy chain	polymer	289	30873.6	1	UniProt (S6B291) Pfam (PF07686) Pfam (PF07654)	Homo sapiens (Human)
7	G (L)	Fab 8I21 light chain	polymer	235	25714.7	1	UniProt (Q6GMX0) Pfam (PF07686) Pfam (PF07654)	Homo sapiens (Human)	Ig kappa chain V-III region CLL,Ig kappa chain V-III region POM
8	H (F)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
9	I, J, K, L, M... (A, B, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	10	Chemie (NAG)

Sequence modifications

A: 1 - 715 (UniProt: Q6SW67)

PDB	External Database	Details
Gly 716	-	expression tag
Ser 717	-	expression tag
Gly 718	-	expression tag
Ser 719	-	expression tag
His 720	-	expression tag
His 721	-	expression tag
His 722	-	expression tag
His 723	-	expression tag
His 724	-	expression tag
His 725	-	expression tag

D: 1 - 214 (UniProt: F5HCP3)

PDB	External Database	Details
Gly 215	-	expression tag
Ser 216	-	expression tag
Glu 217	-	expression tag
Asn 218	-	expression tag
Leu 219	-	expression tag
Tyr 220	-	expression tag
Phe 221	-	expression tag
Gln 222	-	expression tag
Ala 223	-	expression tag
Gly 224	-	expression tag
Trp 225	-	expression tag
Ser 226	-	expression tag
His 227	-	expression tag
Pro 228	-	expression tag
Gln 229	-	expression tag
Phe 230	-	expression tag
Glu 231	-	expression tag
Lys 232	-	expression tag
Gly 233	-	expression tag
Gly 234	-	expression tag
Gly 235	-	expression tag
Ser 236	-	expression tag
Gly 237	-	expression tag
Gly 238	-	expression tag
Gly 239	-	expression tag
Ser 240	-	expression tag
Gly 241	-	expression tag
Gly 242	-	expression tag
Gly 243	-	expression tag
Ser 244	-	expression tag
Trp 245	-	expression tag
Ser 246	-	expression tag
His 247	-	expression tag
Pro 248	-	expression tag
Gln 249	-	expression tag
Phe 250	-	expression tag
Glu 251	-	expression tag
Lys 252	-	expression tag

H: -18 - 111 (PDB: 5VOB)
H: 112 - 227 (UniProt: S6B291)

PDB	External Database	Details
Ser 228	-	expression tag
Ser 229	-	expression tag
Gly 230	-	expression tag
Leu 231	-	expression tag
Glu 232	-	expression tag
Val 233	-	expression tag
Leu 234	-	expression tag
Phe 235	-	expression tag
Gln 236	-	expression tag
Gly 237	-	expression tag
Pro 238	-	expression tag
Leu 239	-	expression tag
Gly 240	-	expression tag
Ser 241	-	expression tag
Ala 242	-	expression tag
Trp 243	-	expression tag
Ser 244	-	expression tag
His 245	-	expression tag
Pro 246	-	expression tag
Gln 247	-	expression tag
Phe 248	-	expression tag
Glu 249	-	expression tag
Lys 250	-	expression tag
Gly 251	-	expression tag
Gly 252	-	expression tag
Gly 253	-	expression tag
Ser 254	-	expression tag
Gly 255	-	expression tag
Gly 256	-	expression tag
Gly 257	-	expression tag
Ser 258	-	expression tag
Gly 259	-	expression tag
Gly 260	-	expression tag
Gly 261	-	expression tag
Ser 262	-	expression tag
Trp 263	-	expression tag
Ser 264	-	expression tag
His 265	-	expression tag
Pro 266	-	expression tag
Gln 267	-	expression tag
Phe 268	-	expression tag
Glu 269	-	expression tag
Lys 270	-	expression tag

L: -19 - 104 (PDB: 5VOB)
L: 105 - 215 (UniProt: Q6GMX0)

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	233303.3
Branched	Number of molecules	1
	Total formula weight	586.5
Non-Polymers*	Number of molecules	10
	Total formula weight	2212.1
All*	Total formula weight	236102.0

*Water molecules are not included.