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5VI8

Structure of a mycobacterium smegmatis transcription initiation complex with an upstream-fork promoter fragment

Replaces:  5TW1
Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(J)
RNA polymerase-binding protein RbpApolymer11413078.71UniProt (A0QZ11)
Pfam (PF13397)
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
2B, C
(A, B)
DNA-directed RNA polymerase subunit alphapolymer35037959.42UniProt (A0QSL8)
Pfam (PF01193)
Pfam (PF01000)
Pfam (PF03118)
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
3D
(C)
DNA-directed RNA polymerase subunit betapolymer1169128680.11UniProt (P60281)
Pfam (PF04563)
Pfam (PF04561)
Pfam (PF04565)
Pfam (PF10385)
Pfam (PF00562)
Pfam (PF04560)
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
4E
(D)
DNA-directed RNA polymerase subunit beta'polymer1317146712.91UniProt (A0QS66)
Pfam (PF04997)
Pfam (PF00623)
Pfam (PF04983)
Pfam (PF05000)
Pfam (PF04998)
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
5F
(E)
DNA-directed RNA polymerase subunit omegapolymer10711544.81UniProt (A0QWT1)
Pfam (PF01192)
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
6G
(F)
RNA polymerase sigma factor SigApolymer46651573.61UniProt (A0QW02)
Pfam (PF00140)
Pfam (PF04542)
Pfam (PF04539)
Pfam (PF04545)
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
7H
(O)
DNA (31-MER)polymer319565.21synthetic construct
8I
(P)
DNA (26-MER)polymer267930.21synthetic construct
9J
(T)
DNA-directed RNA polymerase subunit alphapolymer10011090.01UniProt (A0QSL8)
Pfam (PF03118)
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
10AA, BA, CA, DA, K...
(F, C, D)
SULFATE IONnon-polymer96.112Chemie (SO4)
11EA, N, O, W, X...
(F, C, D)
1,2-ETHANEDIOLnon-polymer62.16Chemie (EDO)
12P, Q
(D)
ZINC IONnon-polymer65.42Chemie (ZN)
13R
(D)
MAGNESIUM IONnon-polymer24.31Chemie (MG)
14FA, GA, HA, IA, JA...
(J, A, B, C, D...)
waterwater18.0109Chemie (HOH)
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains10
Total formula weight456094.4
Non-Polymers*Number of molecules21
Total formula weight1680.3
All*Total formula weight457774.6
*Water molecules are not included.

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PDB entries from 2024-10-30

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