5VI5
Structure of Mycobacterium smegmatis transcription initiation complex with a full transcription bubble
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (O) | DNA (49-MER) | polymer | 50 | 15522.0 | 1 | synthetic construct | ||
| 2 | B (P) | DNA (44-MER) | polymer | 50 | 15393.9 | 1 | synthetic construct | ||
| 3 | C (Q) | RNA (5'-R(*UP*CP*GP*A)-3') | polymer | 4 | 1240.8 | 1 | synthetic construct | ||
| 4 | D, E (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 350 | 37959.4 | 2 | UniProt (A0QSL8) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 5 | F (C) | DNA-directed RNA polymerase subunit beta | polymer | 1169 | 128666.1 | 1 | UniProt (P60281) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 6 | G (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1317 | 146756.9 | 1 | UniProt (A0QS66) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 7 | H (E) | DNA-directed RNA polymerase subunit omega | polymer | 107 | 11544.8 | 1 | UniProt (A0QWT1) Pfam (PF01192) | Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 8 | I (F) | RNA polymerase sigma factor SigA | polymer | 466 | 51573.6 | 1 | UniProt (A0QW02) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) | |
| 9 | J (J) | RNA polymerase-binding protein RbpA | polymer | 114 | 13078.7 | 1 | UniProt (A0QZ11) Pfam (PF13397) | Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) | |
| 10 | K, N, O, P, R... (C, D, F) | SULFATE ION | non-polymer | 96.1 | 6 | Chemie (SO4) | |||
| 11 | L, M (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 12 | Q, T (D, F) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 13 | U, V (C, D) | water | water | 18.0 | 2 | Chemie (HOH) |
Sequence modifications
C: 1 - 1169 (UniProt: P60281)
D: 1 - 1317 (UniProt: A0QS66)
| PDB | External Database | Details |
|---|---|---|
| Asn 238 | Gln 238 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Glu 663 | Ala 663 | conflict |
| Asn 1272 | Gln 1272 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 459695.6 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 831.3 | |
| All* | Total formula weight | 460527.0 |
