5VG6

Crystal structure of D-isomer specific 2-hydroxyacid dehydrogenase from Xanthobacter autotrophicus Py2 in complex with NADPH and MES.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding	polymer	341	37181.4	10	UniProt (A7IIH0) Pfam (PF02826)	Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)
2	BA, K, N, P, R... (J, A, B, C, D...)	NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		745.4	10	Chemie (NDP)
3	AA, CA, L, M, O... (I, J, A, B, C...)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	9	Chemie (MES)
4	DA, EA, FA, GA, HA... (A, B, C, D, E...)	water	water		18.0	2680	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H, I, J: 1 - 319 (UniProt: A7IIH0)

PDB	External Database	Details
Met -21	-	initiating methionine
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Leu -9	-	expression tag
Gly -8	-	expression tag
Thr -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	371814.4
Non-Polymers*	Number of molecules	19
	Total formula weight	9211.3
All*	Total formula weight	381025.8

*Water molecules are not included.