5VE5

Crystal structure of persulfide dioxygenase rhodanese fusion protein with rhodanese domain inactivating mutation (C314S) from Burkholderia phytofirmans in complex with glutathione

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	BpPRF	polymer	377	41604.0	3	UniProt (B2TEQ2) Pfam (PF00753) Pfam (PF00581)	Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)	Beta-lactamase domain protein
2	D, I, L (A, B, C)	FE (III) ION	non-polymer		55.8	3	Chemie (FE)
3	E, J, M (A, B, C)	GLUTATHIONE	non-polymer		307.3	3	Chemie (GSH)
4	F, K, N (A, B, C)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
5	G, O (A, C)	IMIDAZOLE	non-polymer		69.1	2	Chemie (IMD)
6	H (A)	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
7	P, Q, R (A, B, C)	water	water		18.0	189	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 357 (UniProt: B2TEQ2)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ser 314	Cys 314	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	124812.1
Non-Polymers*	Number of molecules	12
	Total formula weight	1484.2
All*	Total formula weight	126296.3

*Water molecules are not included.