5VE5
Crystal structure of persulfide dioxygenase rhodanese fusion protein with rhodanese domain inactivating mutation (C314S) from Burkholderia phytofirmans in complex with glutathione
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C | BpPRF | polymer | 377 | 41604.0 | 3 | UniProt (B2TEQ2) Pfam (PF00753) Pfam (PF00581) In PDB | Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN) | Beta-lactamase domain protein |
2 | A, B, C | FE (III) ION | non-polymer | 55.8 | 3 | Chemie (FE) | |||
3 | A, B, C | GLUTATHIONE | non-polymer | 307.3 | 3 | Chemie (GSH) | |||
4 | A, B, C | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
5 | A, C | IMIDAZOLE | non-polymer | 69.1 | 2 | Chemie (IMD) | |||
6 | A | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 1 | Chemie (PGE) | |||
7 | water | water | 18.0 | 189 | Chemie (HOH) |
Sequence modifications
A, B, C: 1 - 357 (UniProt: B2TEQ2)
PDB | External Database | Details |
---|---|---|
Met -19 | - | expression tag |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Ser 314 | Cys 314 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 124812.1 | |
Non-Polymers* | Number of molecules | 12 |
Total formula weight | 1484.2 | |
All* | Total formula weight | 126296.3 |