5VE4

Crystal structure of persulfide dioxygenase-rhodanese fusion protein with rhodanese domain inactivating mutation (C314S) from Burkholderia phytofirmans

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	BpPRF	polymer	377	41604.0	3	UniProt (B2TEQ2) Pfam (PF00753) Pfam (PF00581)	Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)	Beta-lactamase domain protein
2	D, H, K (A, B, C)	FE (III) ION	non-polymer		55.8	3	Chemie (FE)
3	E, I, L (A, B, C)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
4	F (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5	G (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	J (B)	IMIDAZOLE	non-polymer		69.1	1	Chemie (IMD)
7	M, N, O (A, B, C)	water	water		18.0	88	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 357 (UniProt: B2TEQ2)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ser 314	Cys 314	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	124812.1
Non-Polymers*	Number of molecules	9
	Total formula weight	541.2
All*	Total formula weight	125353.3

*Water molecules are not included.