5VAI

Cryo-EM structure of the activated Glucagon-like peptide-1 receptor in complex with G protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Uncharacterized protein	polymer	461	52581.8	1	UniProt (G1SGD4) Pfam (PF02793) Pfam (PF00002)	Oryctolagus cuniculus (Rabbit)
2	B (P)	Glucagon-like peptide 1	polymer	31	3359.7	1	UniProt (P01275) Pfam (PF00123)	Homo sapiens
3	C (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	380	44370.2	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P54311) Pfam (PF25391)	Rattus norvegicus (Rat)	Transducin beta chain 1
5	E (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7563.8	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (Bovine)	G gamma-I
6	F (N)	nanobody 35	polymer	138	15140.7	1		Lama glama

Sequence modifications

R: 24 - 422 (UniProt: G1SGD4)

PDB	External Database	Details
Met -38	-	expression tag
Lys -37	-	expression tag
Thr -36	-	expression tag
Ile -35	-	expression tag
Ile -34	-	expression tag
Ala -33	-	expression tag
Leu -32	-	expression tag
Ser -31	-	expression tag
Tyr -30	-	expression tag
Ile -29	-	expression tag
Phe -28	-	expression tag
Cys -27	-	expression tag
Leu -26	-	expression tag
Val -25	-	expression tag
Phe -24	-	expression tag
Ala -23	-	expression tag
Asp -22	-	expression tag
Tyr -21	-	expression tag
Lys -20	-	expression tag
Asp -19	-	expression tag
Asp -18	-	expression tag
Asp -17	-	expression tag
Asp -16	-	expression tag
Ala -15	-	expression tag
Ala -14	-	expression tag
Ala -13	-	expression tag
Gly -12	-	expression tag
Gln -11	-	expression tag
Pro -10	-	expression tag
Gly -9	-	expression tag
Asn -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Ala -5	-	expression tag
Phe -4	-	expression tag
Leu -3	-	expression tag
Leu -2	-	expression tag
Ala -1	-	expression tag
Pro 0	-	expression tag
Asn 1	-	expression tag
Arg 2	-	expression tag
Ser 3	-	expression tag
His 4	-	expression tag
Ala 5	-	expression tag
Pro 6	-	expression tag
Gly 7	-	expression tag
Gly 8	-	expression tag
Gly 9	-	expression tag
Gly 10	-	expression tag
Ser 11	-	expression tag
Leu 12	-	expression tag
Glu 13	-	expression tag
Val 14	-	expression tag
Leu 15	-	expression tag
Phe 16	-	expression tag
Gln 17	-	expression tag
Gly 18	-	expression tag
Pro 19	-	expression tag
Gly 20	-	expression tag
Gly 21	-	expression tag
Gly 22	-	expression tag
Ser 23	-	expression tag
Ala 106	Thr 106	conflict
Pro 112	His 112	conflict
Arg 140	Gln 140	conflict

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asp 188	Ala 174	conflict

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	161760.5
All*	Total formula weight	161760.5

*Water molecules are not included.