5V6X

Crystal structure of the tRNA binding domain of Pyrrolysyl-tRNA synthetase mutant (32A NTD) bound to tRNA(Pyl)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Pyrrolysine--tRNA ligase	polymer	109	12609.6	2	UniProt (Q8PWY1)	Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)	Pyrrolysine--tRNA(Pyl) ligase,Pyrrolysyl-tRNA synthetase,PylRS
2	C, D (C, D)	RNA (70-MER)	polymer	72	23114.7	2	GenBank (20986624)	Methanosarcina mazei Go1
3	E, F (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A, B: 1 - 101 (UniProt: Q8PWY1)

PDB	External Database	Details
Met -7	-	expression tag
Gly -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Asn 2	Asp 2	engineered mutation
Asn 3	Lys 3	engineered mutation
Pro 56	Thr 56	engineered mutation
Tyr 62	His 62	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	71448.6
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	71579.4

*Water molecules are not included.