5V27

2.35 angstrom crystal structure of P97V 3-hydroxyanthranilate-3,4-dioxygenase from Cupriavidus metallidurans

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	3-hydroxyanthranilate 3,4-dioxygenase	polymer	201	23081.9	1	UniProt (Q1LCS4) Pfam (PF06052) In PDB	Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)	3-hydroxyanthranilate oxygenase,3-HAO,3-hydroxyanthranilic acid dioxygenase,HAD
2	A	FE (II) ION	non-polymer		55.8	2	Chemie (FE2)
3	A	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
4		water	water		18.0	15	Chemie (HOH)

Sequence modifications

A: 1 - 174 (UniProt: Q1LCS4)

PDB	External Database	Details
Met -26	-	initiating methionine
Gly -25	-	expression tag
Ser -24	-	expression tag
Ser -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Leu -13	-	expression tag
Val -12	-	expression tag
Pro -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Val 97	Pro 97	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	23081.9
Non-Polymers*	Number of molecules	3
	Total formula weight	233.8
All*	Total formula weight	23315.8

*Water molecules are not included.