5UW4

Activated yeast Glycogen Synthase in complex with UDP glucosamine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (C, B, A, D)	Glycogen [starch] synthase isoform 2	polymer	720	81818.6	4	UniProt (P27472) Pfam (PF05693)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
2	E (C)	2-amino-2-deoxy-beta-D-glucopyranose	non-polymer		179.2	1	Chemie (GCS)
3	F, H, J, L (C, B, A, D)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	4	Chemie (UDP)
4	G, I, K, M (C, B, A, D)	6-O-phosphono-alpha-D-glucopyranose	non-polymer		260.1	4	Chemie (G6P)
5	N, O (C, B)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

C, B, A, D: 1 - 700 (UniProt: P27472)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gln 169	Glu 169	engineered mutation
Ser 535	Ala 535	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	327274.5
Non-Polymers*	Number of molecules	9
	Total formula weight	2836.4
All*	Total formula weight	330110.8

*Water molecules are not included.