5USE
5-Se-T4-DNA and native RNA hybrid in complex with RNase H catalytic domain D132N mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | B | RNA (5'-R(*UP*CP*GP*AP*CP*A)-3') | polymer | 6 | 1875.2 | 1 | synthetic construct | ||
| 2 | C | DNA (5'-D(*AP*TP*GP*(T5S)P*CP*G)-3') | polymer | 6 | 1903.2 | 1 | synthetic construct | ||
| 3 | A | Ribonuclease H | polymer | 142 | 16329.5 | 1 | UniProt (Q9KEI9) In PDB | Bacillus halodurans | RNase H |
| 4 | B, A | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 5 | water | water | 18.0 | 32 | Chemie (HOH) |
Sequence modifications
A: 59 - 196 (UniProt: Q9KEI9)
| PDB | External Database | Details |
|---|---|---|
| Gly 55 | - | expression tag |
| Ser 56 | - | expression tag |
| His 57 | - | expression tag |
| Met 58 | - | expression tag |
| Asn 132 | Asp 132 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 20107.9 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 48.6 | |
| All* | Total formula weight | 20156.5 |
