5UPM
Crystal structure of BhGH81 mutant in complex with laminaro-triose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | BH0236 protein | polymer | 753 | 85277.6 | 1 | UniProt (Q9KG76) Pfam (PF03639) Pfam (PF17652) | Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) | |
| 2 | B (B) | beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose | branched | 504.4 | 1 | In PDB GlyTouCan (G00024MO) | |||
| 3 | C (C) | beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-alpha-D-glucopyranose | branched | 504.4 | 1 | In PDB GlyTouCan (G51422BS) | |||
| 4 | D (D) | beta-D-glucopyranose-(1-3)-beta-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900024) GlyTouCan (G36535HU) | beta-laminaribiose | ||
| 5 | E, F, G, H, I... (A) | PHOSPHATE ION | non-polymer | 95.0 | 9 | Chemie (PO4) | |||
| 6 | N, O, P, Q, R... (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 11 | Chemie (EDO) | |||
| 7 | Y (A) | water | water | 18.0 | 899 | Chemie (HOH) |
Sequence modifications
A: 28 - 779 (UniProt: Q9KG76)
| PDB | External Database | Details |
|---|---|---|
| Met 27 | - | initiating methionine |
| Gln 542 | Glu 542 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 85277.6 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1351.2 | |
| Non-Polymers* | Number of molecules | 20 |
| Total formula weight | 1537.5 | |
| All* | Total formula weight | 88166.3 |
